VP8∗P[8] lineages of group A rotaviruses circulating over 20 years in Brazil: Proposal of six different sub-lineages for P[8]-3 clade

► We performed the VP8∗ analysis from P[8] strains to understand this genotype evolution in Brazil. ► The VP8∗ Brazilian strains grouped into three P[8] lineages: P[8]-1, P[8]-2, and P[8]-3. ► During 2001, the co-circulation of these three P[8] lineages was detected in Brazil. ► To date, this is the first description of P[8]-1, P[8]-2 and P[8]-3 at the same time in South America. ► Based on our results we proposed a classification of the P[8]-3 lineage into six sub-lineages.