Multilocus PCR-RFLP profiling in Trypanosoma cruzi I highlights an intraspecific genetic variation pattern

► The multilocus PCR-RFLP strategy allowed us to determine two distinct genetic groups within TcI. ► The groups are related to the transmission cycles (domestic/peridomestic and sylvatic) of Chagas disease. ► Molecular epidemiology of TcI related to symptomatology of Chagas disease in Colombia.