کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5915178 1162789 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Crystallization NoteAcrB et al.: Obstinate contaminants in a picogram scale. One more bottleneck in the membrane protein structure pipeline
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Crystallization NoteAcrB et al.: Obstinate contaminants in a picogram scale. One more bottleneck in the membrane protein structure pipeline
چکیده انگلیسی

Heterologous expression of integral membrane proteins from Helicobacter pylori 26695 in Escherichia coli enabled the identification of 17 candidates for purification and subsequent crystallization. 45% of the purified proteins were contaminated with what was later identified as the multidrug efflux pump (AcrB) of E. coli, and 17% with the succinate dehydrogenase. While additional purification steps ensured removal of succinate dehydrogenase, they failed to remove AcrB completely, leaving picogram amounts present in fractions intended for 3D-crystallization. Two of these targets, the Na+ dependent d-glucose/d-galactose transporter (GluP-HP1174) and the carbon starvation protein A (CstA-HP1168), produced small crystals (<40 μm). Crystals from the GluP preparation diffracted to 4.2 Å resolution and belonged to the rhombohedral space group H32. Subsequent molecular replacement proved that these crystals were derived from a contaminant, the efflux transporter AcrB. This unexpected crystallization of AcrB from picogram amounts was observed in six new conditions. The systematic occurrence of AcrB in membrane preparations stems from the upregulation of its transcription in response to the stress induced by the expression of a selected target. This, along with its tendency to crystallize in the picogram scale, poses a serious concern in membrane protein expression using heterologous hosts harbouring AcrB.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 166, Issue 1, April 2009, Pages 107-111
نویسندگان
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