کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5916215 | 1163375 | 2007 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
Plasmodium bergheiPlasmodium yoeliiCrystallization - بلورسازیcrystallography - بلورنگاری، بلورشناسی یا کریستالوگرافیHeterologous protein expression - بیان پروتئین HeterologousToxoplasma gondii - توکسوپلاسما گوندیMalaria - مالاریاApicomplexa - هاگ داران، اپیکامپلکساPlasmodium knowlesi - پلاسمودیومPlasmodium falciparum - پلاسمودیوم فالسیپارومPlasmodium vivax - پلاسمودیوم ویواکسStructural genomics - ژنومیک سازهCryptosporidium parvum - کریپتوسپوریدیوم پارووم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Parasites from the protozoan phylum Apicomplexa are responsible for diseases, such as malaria, toxoplasmosis and cryptosporidiosis, all of which have significantly higher rates of mortality and morbidity in economically underdeveloped regions of the world. Advances in vaccine development and drug discovery are urgently needed to control these diseases and can be facilitated by production of purified recombinant proteins from Apicomplexan genomes and determination of their 3D structures. To date, both heterologous expression and crystallization of Apicomplexan proteins have seen only limited success. In an effort to explore the effectiveness of producing and crystallizing proteins on a genome-scale using a standardized methodology, over 400 distinct Plasmodium falciparum target genes were chosen representing different cellular classes, along with select orthologues from four other Plasmodium species as well as Cryptosporidium parvum and Toxoplasma gondii. From a total of 1008 genes from the seven genomes, 304 (30.2%) produced purified soluble proteins and 97 (9.6%) crystallized, culminating in 36 crystal structures. These results demonstrate that, contrary to previous findings, a standardized platform using Escherichia coli can be effective for genome-scale production and crystallography of Apicomplexan proteins. Predictably, orthologous proteins from different Apicomplexan genomes behaved differently in expression, purification and crystallization, although the overall success rates of Plasmodium orthologues do not differ significantly. Their differences were effectively exploited to elevate the overall productivity to levels comparable to the most successful ongoing structural genomics projects: 229 of the 468 target genes produced purified soluble protein from one or more organisms, with 80 and 32 of the purified targets, respectively, leading to crystals and ultimately structures from one or more orthologues.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 151, Issue 1, January 2007, Pages 100-110
Journal: Molecular and Biochemical Parasitology - Volume 151, Issue 1, January 2007, Pages 100-110
نویسندگان
Masoud Vedadi, Jocelyne Lew, Jennifer Artz, Mehrnaz Amani, Yong Zhao, Aiping Dong, Gregory A. Wasney, Mian Gao, Tanya Hills, Stephen Brokx, Wei Qiu, Sujata Sharma, Angelina Diassiti, Zahoor Alam, Michelle Melone, Anne Mulichak, Amy Wernimont, James Bray,