کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5916261 1570723 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Affinity maturation of anti-(4-hydroxy-3-nitrophenyl)acetyl antibodies accompanies a modulation of antigen specificity
ترجمه فارسی عنوان
بلوغ وابستگی آنتی بادیهای ضد (4-هیدروکسی-3-نیترفنیل) استیل با مدولاسیون خاصیت آنتی ژن همراه است
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
چکیده انگلیسی


- We analyze fine specificity, heterocliticity, of anti-NP antibodies.
- The antibodies preferentially bind to the phenolate form than to the phenolic form.
- The antibodies become more specific to NP during the process of affinity maturation.

Anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies bearing λ1 chains are known to possess fine specificity, referred to as heterocliticity, which causes these antibodies to bind to hapten analogues such as (4-hydroxy-3-iodo-5-nitrophenyl)acetyl (NIP) and (4-hydroxy-3,5-dinitrophenyl)acetyl (NNP) with higher affinity than to the autologous hapten, NP. They also show preferential binding to the phenolate form of hapten than to the phenolic form. We address here the question of whether affinity maturation accompanies in the fine specificity of these antibodies by analyzing the interaction between NP1-, NIP1-, or NNP1-hen egg lysozyme and anti-NP antibodies that possess different association constants to NP using a surface plasmon resonance biosensor. We measured interactions at various pH values and found that heterocliticity as well as preferential binding to the phenolate form of hapten were most prominent in a germline antibody having immature affinity and that fine specificity becomes less evident, i.e., anti-NP antibodies become more specific to the immunizing antigen, NP during the process of affinity maturation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 70, February 2016, Pages 8-12
نویسندگان
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