کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5917709 1163798 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Human activated CD4+ T lymphocytes increase IL-2 expression by downregulating microRNA-181c
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Human activated CD4+ T lymphocytes increase IL-2 expression by downregulating microRNA-181c
چکیده انگلیسی
MicroRNAs, a large family of small regulatory RNAs, are posttranscriptional gene regulators that bind mRNA in a sequence-specific manner, thereby controlling diverse aspects of cell function, including immune reaction. In this study, we screened and identified a group of differentially expressed miRNAs in naive and activated CD4+ T cells. Among the miRNAs studied, miR-181c was proven to have the potential to regulate CD4+ T cell activation. miR-181c was downregulated in the process of CD4+ T cell activation, and transfection of miR-181c mimics partially repressed the activation of both Jurkat cells and human peripheral blood mononuclear cells (PBMC) CD4+ T cells. We further showed that miR-181c can bind to the IL-2 3′ UTR and repress its expression by inhibiting translation. Moreover, miR-181c mimics reduced activated CD4+ T cell proliferation. Taken together, our results show that miR-181c serves as a negative regulator that modulates the activation of CD4+ T cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 48, Issue 4, January 2011, Pages 592-599
نویسندگان
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