کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5918005 1163817 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An in vivo functional genetic screen for suppressors of the Rag1−/− T-cell defect
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
An in vivo functional genetic screen for suppressors of the Rag1−/− T-cell defect
چکیده انگلیسی
Functional genetic screens on mutant backgrounds have been successfully used in lower organisms to investigate biological processes. However, few identical screens have been performed in mice. Recombinase activating gene-1 deficient (Rag1−/−) mice have a severe T-cell developmental block owing to lack of rearrangement of their T-cell receptor (TCR) genes. Using a retroviral cDNA library derived from wild-type embryonic thymocytes we performed a suppressor screen in Rag1−/− hematopoietic cells and recovered TCRβ. This is the first demonstration that targeted genetic screens are feasible using transduced primary cells in vivo. Consequently, this technique can be used to interrogate multiple blood lineages using diverse hematopoietic mouse mutants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 45, Issue 3, February 2008, Pages 682-689
نویسندگان
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