کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5918340 | 1163858 | 2008 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Complex regulation of human cathelicidin gene expression: Novel splice variants and 5â²UTR negative regulatory element
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
TNFIL-LPSORFsplice variants - انواع اتصالاتInnate immunity - ایمنی ذاتیinterferon - اینترفرونIFN - اینترفرون هاinterleukin - اینترلوکینReverse transcriptase - ترانس کریپتاز معکوس یا وارونویسrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدGene regulation - تنظیم ژنRegulatory elements - عناصر تنظیمیtumor necrosis factor - فاکتور نکروز تومورopen reading frame - قاب خواندن بازlipopolysaccharide - لیپوپلی ساکاریدRace - مسابقهUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازPromoter - پروموترAntimicrobial peptide - پپتیدهای ضدمیکروبی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Complex regulation of human cathelicidin gene expression: Novel splice variants and 5â²UTR negative regulatory element Complex regulation of human cathelicidin gene expression: Novel splice variants and 5â²UTR negative regulatory element](/preview/png/5918340.png)
چکیده انگلیسی
Cationic antimicrobial peptides play important roles in host defense, linking innate and adaptive immunity. hCAP18, the only human antimicrobial cathelicidin, consists of a conserved N-terminal cathelin-like domain and a C-terminal peptide, LL-37. Expression is regulated during myeloid differentiation, and tightly controlled during infection and inflammation, suggesting active regulation. Using 5â² RACE (rapid amplification of cDNA ends), multiple transcription initiation sites were identified, as well as new splice variants leading to novel augmentations of hCAP18 amino acid composition in bone marrow but not peripheral blood neutrophils. Having expressed hCAP18 promoter constructs in cell lines, we found that full-length (â1739) and truncated (â978) promoter constructs had lower luciferase activities than 5â²UTR deletion constructs. Transient transfection of progressively deleted constructs in the non-permissive K562 cell line led us to identify a negative regulatory element within the 53Â bp immediately upstream of the ATG of hCAP18. Additionally, transient transfection of 5â² deletion constructs identified a positive regulatory element within the 101 bases 5â² of promoter sequence containing two GT-boxes. Negative and positive regulatory elements within the hCAP18 gene promoter provide new insights into the possible molecular basis of myeloid gene expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 45, Issue 1, January 2008, Pages 204-217
Journal: Molecular Immunology - Volume 45, Issue 1, January 2008, Pages 204-217
نویسندگان
Houda Zghal Elloumi, Steven M. Holland,