Stimuli affecting zebrafish (Danio rerio) behavior in the light/dark preference test

► Light/Dark preference in zebrafish depends on the stimuli used. ► When actively swimming, zebrafish prefer an open/transparent chamber to black or white, but show little preference for black over white. ► Zebrafish choose to freeze in black rather than white. ► Low doses of ethanol increase locomotor activity, but reduce the amount of activity in white.