Human group X secreted phospholipase A2 induces dendritic cell maturation through lipoprotein-dependent and -independent mechanisms

ObjectiveIncreased secreted phospholipase A2 (sPLA2) activity has been documented in several inflammatory disorders. Among sPLA2s, the human group X (hGX)-sPLA2 has the highest catalytic activity towards phosphatidylcholine (PC), the major phospholipid of cell membranes and blood lipoproteins. hGX-sPLA2 has been detected in human atherosclerotic lesions, indicating that sPLA2s are an important link between lipids and inflammation, both involved in atherosclerosis. The presence of dendritic cells (DC), the most potent antigen presenting cells, in atherosclerotic lesions has raised the question about their role in disease progression.Methods and resultsIn this study, we show that hGX-sPLA2-treated LDL induces human monocyte-derived DC maturation, resulting in a characteristic mature DC phenotype and enhanced DC ability to activate IFNγ secretion from T cells. hGX-sPLA2 phospholipolysis of LDL produces high levels of lipid mediators, such as lysophosphatidylcholine (LPC) and free fatty acids (FFAs), which also modulate DC maturation. The major molecular species of LPC containing a palmitic or stearic acid esterified in the sn-1 position induce DC maturation, whereas the FFAs can positively or negatively modulate DC maturation depending on their nature. hGX-sPLA2 added alone can also activate DC in vitro through the hydrolysis of the DC membrane phospholipids leading, however, to a different cytokine profile secretion pattern than the one observed with hGX-sPLA2-phospholipolysed LDL.ConclusionhGX-sPLA2 secreted in inflamed tissues can contribute to local DC maturation, resulting in pro-Th1 cells, through the production of various lipid mediators from hydrolysis of either LDL and/or cell plasma membrane.

► Phospholipolyzed LDL induces DC maturation resulting in pro-Th1 cells. ► hGX-sPLA2 can directly activate DC through membrane phospholipids hydrolysis. ► LPC and FFA modulation of DC maturation depends on their fatty acid content.