کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5948460 | 1172379 | 2012 | 7 صفحه PDF | دانلود رایگان |
ObjectivesTo study the effects of reduced lipoic acid gene expression on diabetic atherosclerosis in apolipoprotein E null mice (Apoeâ/â).Methods and resultsHeterozygous lipoic acid synthase gene knockout mice (Lias+/â) crossed with Apoeâ/â mice were used to evaluate the diabetic effect induced by streptozotocin on atherosclerosis in the aortic sinus of the heart. While diabetes markedly increased atherosclerotic plaque size in Apoeâ/â mice, a small but significant effect of reduced expression of lipoic acid gene was observed in diabetic Lias+/âApoeâ/â mice. In the aortic lesion area, the Lias+/âApoeâ/â mice exhibited significantly increased macrophage accumulation and cellular apoptosis than diabetic Lias+/+Apoeâ/â littermates. Plasma glucose, cholesterol, and interleukin-6 were also higher. These abnormalities were accompanied with increased oxidative stress including a decreased ratio of reduced glutathione/oxidized glutathione in erythrocytes, increased systemic lipid peroxidation, and increased Gpx1 and MCP1 gene expression in the aorta.ConclusionsDecreased endogenous lipoic acid gene expression plays a role in development of diabetic atherosclerosis. These findings extend our understanding of the role of antioxidant in diabetic atherosclerosis.
⺠We evaluate effects of reduced lipoic acid synthase gene expression on diabetic atherosclerosis. ⺠Apolipoprotein E null mice were crossed with Lias antioxidant model and then induced to diabetes. ⺠Reduced endogenous lipoic acid capacity plays a role in development of diabetic atherosclerosis. ⺠These findings extend our understanding of the role of antioxidant in diabetic atherosclerosis.
Journal: Atherosclerosis - Volume 223, Issue 1, July 2012, Pages 137-143