Mechanistic basis of excitation-contraction coupling in human pluripotent stem cell-derived ventricular cardiomyocytes revealed by Ca2+ spark characteristics: Direct evidence of functional Ca2+-induced Ca2+ release

Taken collectively, our findings provide the first lines of direct evidence that hESC-VCMs have functional Ca2+-induced Ca2+ release. However, the sarcoplasmic reticulum is leaky and without a mature terminating mechanism in early development.