کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5977086 | 1576213 | 2013 | 8 صفحه PDF | دانلود رایگان |
The discovery of peripheral circulating cells that contribute to vasculogenesis and endothelial repair was one of the most fascinating breakthroughs in the domain of vascular research during the last two decades. The population of vasculogenic cells however, is heterogeneous and can be analyzed using different approaches including in vitro culture and flow cytometry. Circulating CD34+/KDR+/CD45dim endothelial progenitor cells (EPC) have a great potential as biomarkers in various cardiovascular diseases. With the expanding interest in this field, the development of standardized protocols is critical.In this review we describe in detail the pre-analytical and analytical factors that should be taken into account when quantifying CD34+/KDR+/CD45dim EPC using flow cytometry. Moreover, technical suggestions in order to enhance accuracy and reproducibility of this enumeration are provided.
Journal: International Journal of Cardiology - Volume 167, Issue 5, 1 September 2013, Pages 1688-1695