کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
599673 1454286 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Conformational changes of DNA in the presence of 12-s-12 gemini surfactants (s = 2 and 10). Role of the spacer's length in the interaction surfactant-polynucleotide
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Conformational changes of DNA in the presence of 12-s-12 gemini surfactants (s = 2 and 10). Role of the spacer's length in the interaction surfactant-polynucleotide
چکیده انگلیسی


• The presence of the dimeric (gemini) cationic surfactants in calf thymus DNA solutions induces a partial condensation of the polynucleotide.
• Micellar aggregates act as multivalent ions which lead to the formation of a nucleation center in the DNA strands.
• Spacer length of dimeric surfactants plays a main role in the condensation process of DNA.
• The breaking of the partial condensation and the formation of a new polynucleotide state happen for the higher [surfactant]/[DNA] values.
• Kinetic measurements can also be used to determine the structural change of a polymer.

A multifaceted study on the interaction of calf-thymus DNA with two different cationic gemini surfactants alkanediyl-α-ω-bis(dodecyldimethyl-amonium)bromide, 12-s-12,2Br− (with s = 2, G2, and 10, G10) was carried out. The measurements were done at different molar ratios X = [surfactant]/[DNA]. Results show two different conformational changes in DNA: a first compaction of the polynucleotide corresponding to a partial conformational (not total) change of DNA from an extended coil state to a globular state that happens at the lower molar ratio X. A second change corresponds to a breaking of the partial condensation, that is, the transition from the compacted state to a new more extended conformation (for the higher X values) different to the initial extension. According to circular dichroism spectra and dynamic light scattering measurements, this new state of DNA seems to be similar to a ψ-phase. Measurements confirm that interactions involved in the compaction are different to those previously obtained for the analog surfactant CTAB. X values at which the conformational changes happen depend on the length of the spacer in the surfactant along with the charge of the polar heads.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 118, 1 June 2014, Pages 90–100
نویسندگان
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