Modification of surface and enzymatic properties of Achromobacter denitrificans and Stenotrophomonas maltophilia in association with diesel oil biodegradation enhanced with alkyl polyglucosides

• Alkyl polyglucosides are effective surface active agents for bioremediation.
• Surfactants concentration influence on biodegradation only for A. denitrificans.
• Cell surface hydrophobicity changes are dependent on the kind of microorganisms.
• Presence of diesel oil induces the production of oxidizing enzymes.

The article concerns the influence of selected alkyl polyglucosides on biodegradation, cell surface and enzymatic properties of Stenotrophomonas maltophilia and Achromobacter denitrificans.The biodegradation of diesel oil depends on several factors including type and the amount of surfactant as well as bacterial genera used in the process. Nevertheless, a careful selection of these variables must be made as some bacterial strains prefer to use surfactants as their carbon source. This leads to the lowered biodegradation of diesel oil as can be observed for the tested S. maltophilia strain. Alkyl polyglucosides influenced the cell surface properties of both of the tested strains in slightly different ways. Especially for A. denitrificans, for which the hydrophobicity increased with concentration of both – Lutensol GD 70 and Glucopon 215 in diesel oil–surfactant systems. Moreover, judging by the efficiency of biodegradation, the most effective process was observed in the presence of Lutensol GD 70 (240 and 360 mg L−1) with biodegradation rising from 32% (without surfactant) to 68%. No such relation was observed for S. maltophilia.

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