Platonin mitigates acute lung injury in haemorrhagic shock rats

Aim of the studyEnhanced oxidative stress and inflammatory response are crucial in mediating the development of acute lung injury induced by haemorrhagic shock with resuscitation. Platonin, a potent antioxidant, possesses potent anti-inflammation capacity. We sought to elucidate whether platonin could mitigate acute lung injury in haemorrhagic shock/resuscitation rats.MethodsSeventy-two adult male rats were randomized to receive haemorrhagic shock/resuscitation (HS), HS plus platonin (10, 50, or 100 μg/kg intravenous injection immediately after resuscitation), sham instrumentation (Sham), or Sham plus platonin (100 μg/kg) (n = 12 in each group). Haemorrhagic shock was induced by blood drawing and mean blood pressure was maintained at 40-45 mmHg for 120 min. Then, resuscitation was achieved by shed blood/saline mixtures re-infusion. After monitoring for another 8 h, rats were sacrificed.ResultsArterial blood gas and histological findings, in concert with assays of leukocyte infiltration (polymorphonuclear leukocytes/alveoli ratio and myeloperoxidase activity) and lung water content (wet/dry weight ratio), confirmed that haemorrhagic shock/resuscitation caused significant lung injury. Significant increases in concentrations of inflammatory molecules (chemokine, cytokine, and prostaglandin E2) as well as nitric oxide and malondialdehyde in lung tissues confirmed that haemorrhagic shock/resuscitation elicited inflammatory response and imposed oxidative stress in rats. Platonin at the dosages of 50 and 100 μg/kg, but not 10 μg/kg, significantly attenuated the inflammatory response and oxidative stress induced by haemorrhagic shock/resuscitation. Most important, platonin at the dosages of 50 and 100 μg/kg, but not 10 μg/kg, significantly mitigated the lung injury induced by haemorrhagic shock/resuscitation.ConclusionsPlatonin mitigates acute lung injury in haemorrhagic shock/resuscitation rats.