Solid-phase PEGylation of an immobilized protein cage on polyelectrolyte multilayer

- Analysis of solid-phase PEGylation using immobilized protein cages by QCM.
- The percentage of surface covered with PEG was controlled by PEGylation conditions.
- Immobilized protein cages provide a spatially restricted reaction field for PEGylation.
- A mechanism was proposed to illustrate the solid-phase PEGylation of protein cages.
- The protein cage surface covered with PEG alters the interaction with other proteins.