کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
600459 1454303 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Formation and retention of staphylococcal biofilms on DLC and its hybrids compared to metals used as biomaterials
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Formation and retention of staphylococcal biofilms on DLC and its hybrids compared to metals used as biomaterials
چکیده انگلیسی

Staphylococcus epidermidis and Staphylococcus aureus cause most of the implant-related infections. Antibiotic treatment often fails and cure requires surgical intervention. It was hypothesized that biomaterial coatings resistant to biofilms offer a preventive option. Physical vapour deposited diamond-like carbon (DLC) and its polytetrafluoroethylene (DLC-PTFE-h) and polydimethylsiloxane (DLC-PDMS-h) hybrids were compared to titanium (Ti), tantalum (Ta) and chromium (Cr) thin films on silicon wafers for their resistance against formation and/or retention of biofilms produced by S. epidermidis and S. aureus in vitro. Sample surfaces were characterized for surface topography, contact angle and zeta-potential, because such properties might affect the biofilm. Biofilm was stained using calcofluor white and analysed in fluorescence microscopy using morphometry. Sixteen hour incubation was selected in pilot tests; at this checkpoint Ti, Ta, Cr and DLC-PDMS-h were almost fully covered by biofilm, but DLC and DLC-PTFE-h were only partially biofilm coated by S. epidermidis (88 ± 26%, p < 0.001 and 56 ± 39%, p < 0.001, respectively) or S. aureus (81 ± 24%, p < 0.001 and 51 ± 26%, p < 0.001, respectively). DLC and its PTFE hybrid offer a potential biofilm hostile surface coating for implants and medical devices. This ability to resist biofilm formation and attachment could not be explained by only one factor, but it seems to be related to a combination of various properties, with electrokinetic streaming potential and protein coating being particularly important for its outcome.

Figure optionsDownload as PowerPoint slideHighlights
► Formation and/or retention of staphylococcal biofilms is evaluated on biomaterials.
► Surface roughness, contact angle and zeta-potential values of surfaces are measured.
► Diamond-like carbon polytetrafluoroethylene hybrid (DLC-PTFE-h) is superior in test.
► DLC and its PTFE hybrid are biofilm hostile surface coatings for medical devices.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 101, 1 January 2013, Pages 290–297
نویسندگان
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