Interactions between ionic liquid surfactant [C12mim]Br and DNA in dilute brine

Interactions between ionic liquid surfactant [C12mim]Br and DNA in dilute brine were investigated in terms of various experimental methods and molecular dynamics (MD) simulation. It was shown that the aggregation of [C12mim]Br on DNA chains is motivated not only by electrostatic attractions between DNA phosphate groups and [C12mim]Br headgroups but also by hydrophobic interactions among [C12mim]Br alkyl chains. Isothermal titration calorimetry analysis indicated that the [C12mim]Br aggregation in the presence and absence of DNA are both thermodynamically favored driven by enthalpy and entropy. DNA undergoes size transition and conformational change induced by [C12mim]Br, and the charges of DNA are neutralized by the added [C12mim]Br. Various microstructures were observed such as DNA with loose coil conformation in nature state, necklace-like structures, and compact spherical aggregates. MD simulation showed that the polyelectrolyte collapses upon the addition of oppositely charged surfactants and the aggregation of surfactants around the polyelectrolyte was reaffirmed. The simulation predicted the gradual neutralization of the negatively charged polyelectrolyte by the surfactant, consistent with the experimental results.

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► Aggregation of [C12mim]Br on DNA is led by electrostatic and hydrophobic interactions.
► Aggregation of [C12mim]Br is thermodynamically favored driven by enthalpy and entropy.
► DNA conformation and the charges carried by DNA can be modulated by [C12mim]Br.
► MD simulation emphasizes the interaction process and affirms the experimental results.