Immobilization of Candida antarctica A and Thermomyces lanuginosus lipases on cotton terry cloth fibrils using polyethyleneimine

► Lipases from Candida antarctica A and Thermomyces lanuginosus were immobilized on cotton cloth by polyethyleneimine. ► The highest amount of precipitated enzyme was obtained at PEI to enzyme weight ratio of 1/20-1/40 for C. antarctica A and T. lanuginosus. ► C. antarctica A and T. lanuginosus lipases were successfully immobilized as 200 mg/g support and 180 mg/g support, respectively. ► Immobilized lipases exhibited that they could be stored at room temperature with a little activity lost during 28 days.