کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
600821 1454313 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Application of albumin-grafted scaffolds to promote neocartilage formation
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Application of albumin-grafted scaffolds to promote neocartilage formation
چکیده انگلیسی

This study investigates the capacity of albumin-grafted biomaterials as tissue engineering scaffolds to regenerate cartilaginous components. Porcine knee chondrocytes were seeded and cultivated in porous ternary matrix consisting of polyethylene oxide, chitin, and chitosan with surface albumin. The results revealed that the quantity of albumin did not affect the viability of porcine knee chondrocytes in the constructs. However, a high grafting concentration of albumin favored the adhesion of porcine knee chondrocytes on the scaffolding pore surface. After cultivation over 4 weeks, an increase in the concentration of albumin enhanced the quantities of porcine knee chondrocytes, glycosaminoglycans, and collagen in the constructs. The histological staining of porcine knee chondrocytes showed an active chondrocytic growth in the albumin-grafted constructs. In addition, the safranin-O staining indicated that the surface albumin could stabilize the secretion of glycosaminoglycans. Moreover, the immunochemical staining against type II collagen exhibited a regular production of collagen by phenotypic porcine knee chondrocytes in the constructs. Albumin-grafted polyethylene oxide/chitin/chitosan scaffolds can be a promising biomimetic substrate in neocartilage formation.

Figure optionsDownload as PowerPoint slideHighlights
► The albumin quantity in the constructs does not affect the viability of porcine knee chondrocytes.
► A high albumin concentration favors the adhesion of porcine knee chondrocytes on the pore surface.
► A high albumin concentration enhances the formation of neocartilage.
► H&E, safranin-O, and type II collagen staining show the regeneration of cartilaginous components.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 91, 1 March 2012, Pages 296–301
نویسندگان
, , , , ,