Spectroscopic and microscopic characterization of biosensor surfaces with protein/amino-organosilane/silicon structure

► Biorecognition protein layers created on silicon modified with amino-organosilanes. ► Complex impact of blocking of non-specific binding and immunoreaction with antibody. ► Lateral nanostructures not affected by blocking but coarsen due to specific binding. ► Protein surface coverage, not increased due to blocking, rises after immunoreaction. ► Polar functional groups orient towards substrate for all protein layers.