کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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601006 | 879929 | 2011 | 7 صفحه PDF | دانلود رایگان |
The interaction of the amphiphilic drugs, i.e., amitriptyline hydrochloride (AMT) and promethazine hydrochloride (PMT), with serum albumins (i.e., human serum albumin (HSA) and bovine serum albumin (BSA)), has been examined by the various spectroscopic techniques, like fluorescence, UV–vis, and circular dichroism (CD). Fluorescence results indicate that in case of HSA–drug complexes the quenching of fluorescence intensity at 280 nm is less effective as compared to at 295 nm while in case of BSA–drug complexes both have almost same effect and for most of drug–serum albumin complexes there is only one independent class of binding. For all drug–serum albumin complexes the quenching rate constant (Kq) values suggest the static quenching procedure. The UV–vis results show that the change in protein conformation of PMT–serum albumin complexes was more prominent as compared to AMT–serum albumin complexes. The CD results also explain the conformational changes in the serum albumins on binding with drugs. The increase in α-helical structure for AMT–serum albumin complexes is found to be more as compared to PMT–serum albumin complexes. Hence, the various spectroscopic techniques provide a quantitative understanding of the binding of amphiphilic drugs with serum albumins.
Variation of % helical structure of serum albumins with the varying concentration of drugs.Figure optionsDownload as PowerPoint slideHighlights
► Binding capacity and structure of the complexes between serum albumins and amphiphilic drugs are of particular interest in order to elucidate the means by which ligand affinity is regulated.
► Biophysical interactions play important role in drug transport and storage.
► Although there is a vast literature on the interaction of serum albumins with the surfactants, report on the interaction study of amphiphilic drugs with serum albumins are scarce.
► Keeping this in mind, the interaction of the amphiphilic drugs with the serum albumins is seen.
Journal: Colloids and Surfaces B: Biointerfaces - Volume 87, Issue 2, 15 October 2011, Pages 447–453