کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
602456 879978 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Surface characterization of immunosensor conjugated with gold nanoparticles based on cyclic voltammetry and X-ray photoelectron spectroscopy
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Surface characterization of immunosensor conjugated with gold nanoparticles based on cyclic voltammetry and X-ray photoelectron spectroscopy
چکیده انگلیسی
This investigation describes the surface characterization of rabbit immunoglobulin G (IgG) conjugated with gold nanoparticles. Goat anti-rabbit immunoglobulin G tagged with 5 nm gold nanoparticles was applied to detect the IgG. Then, the autocatalyzed deposition of Au3+ onto the surface of anti-IgGAu increased the surface area per gold nanoparticle. The immobilization chemistries and the atomic concentrations of Au4f, P2p, S2p, C1s, N1s and O1s of the resulting antibody-modified Au electrodes were determined by X-ray photoelectron spectroscopy (XPS). The sulfur that is involved in the cysteamine binding and the enlargement of the gold nanoparticles are identified using cyclic voltammetry. The results reveal that the surface area per gold particle, following the autocatalyzed deposition Au3+ on the surface of anti-IgGAu, was approximately seven times higher than that before deposition.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 68, Issue 2, 1 February 2009, Pages 130-135
نویسندگان
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