کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
602469 | 879978 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A study on the interactions of Aurein 2.5 with bacterial membranes
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
شیمی کلوئیدی و سطحی
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چکیده انگلیسی
Aurein 2.5 (GLFDIVKKVVGAFGSL-NH2) is an uncharacterised antimicrobial peptide. At an air/water interface, it exhibited strong surface activity (maximal surface pressure 25 mN mâ1) and molecular areas consistent with the adoption of α-helical structure orientated either perpendicular (1.72 nm2 moleculeâ1) or parallel (3.6 nm2 moleculeâ1) to the interface. Aurein 2.5 was strongly antibacterial, exhibiting a minimum inhibitory concentration (MIC) of 30 μM against Bacillus subtilis and Escherichia coli. The peptide induced maximal surface pressure changes of 9 mN mâ1 and 5 mN mâ1, respectively, in monolayers mimicking membranes of these organisms whilst compression isotherm analysis of these monolayers showed ÎGMix > 0, indicating destabilisation by Aurein 2.5. These combined data suggested that toxicity of the peptide to these organisms may involve membrane invasion via the use of oblique orientated α-helical structure. The peptide induced strong, comparable maximal surface changes in monolayers of DOPG (7.5 mN mâ1) and DOPE monolayers (6 mN mâ1) suggesting that the membrane interactions of Aurein 2.5 were driven by amphiphilicity rather than electrostatic interaction. Based on these data, it was suggested that the differing ability of Aurein 2.5 to insert into membranes of B. subtilis and E. coli was probably related to membrane-based factors such as differences in lipid packing characteristics. The peptide was active against both sessile E. coli and Staphylococcus aureus with an MIC of 125 μM. The broad-spectrum antibacterial activity and non-specific modes of membrane action used by Aurein 2.5 suggested use as an anti-biofilm agent such as in the decontamination of medical devices.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 68, Issue 2, 1 February 2009, Pages 225-230
Journal: Colloids and Surfaces B: Biointerfaces - Volume 68, Issue 2, 1 February 2009, Pages 225-230
نویسندگان
Sarah R. Dennison, Leslie H.G. Morton, Andrea J. Shorrocks, Frederick Harris, David A. Phoenix,