کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
602850 | 879997 | 2006 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The analysis of serum effects on structure, size and toxicity of DDAB–DOPE and DC-Chol–DOPE lipoplexes contributes to explain their different transfection efficiency The analysis of serum effects on structure, size and toxicity of DDAB–DOPE and DC-Chol–DOPE lipoplexes contributes to explain their different transfection efficiency](/preview/png/602850.png)
The effect of serum on structural properties of dimethyl-dioctadecyl-ammonium bromide (DDAB)–1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) liposomes and DDAB–DOPE/DNA lipoplexes has been investigated by energy dispersive X-ray diffraction (EDXD) technique, at different cationic lipid/DNA weight ratios (ρ). The role of serum on the size of lipoplexes has also been studied by dynamic light scattering. Lipoplex transfection efficiency (TE) as a function of ρ, and lipoplex toxicity to C6 rat glioma cells have been evaluated in Dulbecco's Modified Eagle Medium (DMEM) with and without serum. A multi-parametric analysis concerning the role of size, structure and cytotoxicity on transfection efficiency contributes to explain the experimental observation that 3β-[N-(N′,N′-dimethylaminoethane)carbamoyl]-cholesterol (DC-Chol)–DOPE/DNA transfect C6 cells better than DDAB–DOPE/DNA lipoplexes.
Journal: Colloids and Surfaces B: Biointerfaces - Volume 53, Issue 2, 1 December 2006, Pages 187–192