Multiscale vision model highlights spontaneous glial calcium waves recorded by 2-photon imaging in brain tissue

► We modify multiscale vision model (MVM) to process stacks of fluorescent images. ► The modified MVM was applied to characterization of spontaneous glial calcium waves. ► The method is more sensitive and robust than ad hoc smooth-and-threshold approach. ► We demonstrate non-linear nature of glial calcium waves (GCWs). ► Blood vessels reshape GCWs and are associated with generation of secondary GCWs.