Quantification of blood flow and volume in arterioles and venules of the rat cerebral cortex using functional micro-ultrasound

► Functional micro-ultrasound imaging (fMUS) is a new modality for hemodynamic imaging. ► fMUS enables quantification of relative CBV and CBF throughout the rat brain. ► Color Doppler ultrasound imaging identifies cortical arterioles and venules. ► rCBV is lower and flow rate is higher in cortical arterioles than in cortical venules. ► The CBF-CBV relationship is different in cortical vs. subcortical gray matter.