کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6102739 | 1211119 | 2014 | 19 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: ReviewStrategies for immortalization of primary hepatocytes ReviewStrategies for immortalization of primary hepatocytes](/preview/png/6102739.png)
SummaryThe liver has the unique capacity to regenerate in response to a damaging event. Liver regeneration is hereby largely driven by hepatocyte proliferation, which in turn relies on cell cycling. The hepatocyte cell cycle is a complex process that is tightly regulated by several well-established mechanisms. In vitro, isolated hepatocytes do not longer retain this proliferative capacity. However, in vitro cell growth can be boosted by immortalization of hepatocytes. Well-defined immortalization genes can be artificially overexpressed in hepatocytes or the cells can be conditionally immortalized leading to controlled cell proliferation. This paper discusses the current immortalization techniques and provides a state-of-the-art overview of the actually available immortalized hepatocyte-derived cell lines and their applications.
Journal: Journal of Hepatology - Volume 61, Issue 4, October 2014, Pages 925-943