کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6109783 1211214 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research ArticleA new NMR-based metabolomics approach for the diagnosis of biliary tract cancer
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی بیماری‌های گوارشی
پیش نمایش صفحه اول مقاله
Research ArticleA new NMR-based metabolomics approach for the diagnosis of biliary tract cancer
چکیده انگلیسی

Background & AimsBiliary tract cancer is highly lethal at presentation, with increasing mortality worldwide. Current diagnostic measures employing multiple criteria such as imaging, cytology, and serum tumor markers are not satisfactory, and a new diagnostic tool is needed. Because bile is a cognate metabolite-rich bio-fluid in the biliary ductal system, we tested a new metabolomic approach to develop an effective diagnostic tool.MethodsBiles were collected prospectively from patients with cancer (n = 17) or benign biliary tract diseases (n = 21) with percutaneous or endoscopic methods. Nuclear magnetic resonance spectra (NMR) of these biles were analyzed using orthogonal partial least square discriminant analysis (OPLS-DA).ResultsThe metabolomic 2-D score plot showed good separation between cancer and benign groups. The contributing NMR signals were analyzed using a statistical TOCSY approach. The diagnostic performance assessed by leave-one-out analysis exhibited 88% sensitivity and 81% specificity, better than the conventional markers (CEA, CA19-9, and bile cytology).ConclusionThe NMR-based metabolomics approach provides good performance in discriminating cancer and benign biliary duct diseases. The excellent predictability of the method suggests that it can, at least, augment the currently available diagnostic approaches.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Hepatology - Volume 52, Issue 2, February 2010, Pages 228-233
نویسندگان
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