کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6115422 1215903 2016 18 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and validation of a point-of-care test for detecting hantavirus antibodies in human and rodent samples
ترجمه فارسی عنوان
توسعه و اعتبار یک آزمون مراقبت از نقطه برای تشخیص آنتیبادیهای هانتویروس در نمونه های انسانی و جوندگان
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
چکیده انگلیسی
Hantaviruses are etiologic agents of a zoonotic disease transmitted mainly from wild rodents to humans, causing Hemorrhagic Fever with Renal Syndrome in Eurasia and the Hantavirus Cardiopulmonary Syndrome in the Americas (HCPS), reaching a lethality rate of 40% in Brazil. Hantavirus diagnostic and seroprevalence are often based on the presence of IgM and IgG antibodies against the virus. Here we propose a rapid test assay able to identify hantavirus antibodies with sensibility and specificity similar to ELISA assays. We analyzed five groups of samples, including healthy human population and small mammals of endemic areas, suspected cases of HCPS, patients with non-related infections and a serum panel from a different geographical region. The test presented good rates of sensibility (87-100%) and specificity (97-100%) for all groups, being a promising tool suitable for both rodent and human hantavirus epidemiological surveys.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 85, Issue 3, July 2016, Pages 323-327
نویسندگان
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