کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6115455 | 1215904 | 2015 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Susceptibility testing of Mycobacterium abscessus by isothermal microcalorimetry
ترجمه فارسی عنوان
تست حساسیت میکروسکوپیک اسپکتسوس توسط میکروکالوریمتری های ایزوترمی
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کلمات کلیدی
میکوباکتریوم میکوباکتریوم آبسه میکروکالوریمتری، تست حساسیت، آنتی بیوتیک ها،
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
چکیده انگلیسی
We evaluated a new method for susceptibility testing of a rapidly growing mycobacterium using real-time measurement of heat (microcalorimetry). MICs of 2 clinical Mycobacterium abscessus isolates were determined by microbroth dilution and E-test. For microcalorimetry, Middlebrook-7H10 agar + 10% oleic acid-albumin-dextrose-catalase, containing amikacin, clarithromycin, linezolid, and ciprofloxacin was inoculated with ~105 CFU/mL. Heat production was measured at 37 °C for 72 h. Minimal heat inhibition concentration (MHIC) was defined as the lowest antibiotic concentration inhibiting growth-related heat production. Growth of M. abscessus was detected after a median of 16.5 h (range, 8.5-26.9 h). Heat detection was proportionally delayed with increasing concentration of antibiotics. MHICs for the tested strains were 16 to >16 mg/L for amikacin, >8 mg/L for clarithromycin, 4 to >16 mg/L for ciprofloxacin, 24 to >32 mg/L for linezolid. MHICs were in agreement within two 2-fold dilutions with conventional MICs. Microcalorimetry may accelerate antimicrobial susceptibility testing in mycobacteria and provide additional real-time information on the drug effect.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 83, Issue 2, October 2015, Pages 139-143
Journal: Diagnostic Microbiology and Infectious Disease - Volume 83, Issue 2, October 2015, Pages 139-143
نویسندگان
Noémie Boillat-Blanco, Ulrika Furustrand Tafin, Katia Jaton, Andrej Trampuz,