کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6115753 | 1215928 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid detection of Enterococcus spp. direct from blood culture bottles using Enterococcus QuickFISH Method: a multicenter investigation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The performance of a diagnostic method for detection and identification of Enterococcus spp. directly from positive blood culture was evaluated in a clinical study. The method, Enterococcus QuickFISH BC, is a second-generation peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH) test, which uses a simplified, faster assay procedure. The test uses fluorescently labeled PNA probes targeting 16S rRNA to differentiate Enterococcus faecalis from other Enterococcus spp. by the color of the cellular fluorescence. Three hundred fifty-six routine blood culture samples were tested; only 2 discordant results were recorded. The sensitivities for detection of Enterococcus faecalis and non-faecalis Enterococcus were 100% (106/106) and 97.0% (65/67), respectively, and the combined specificity of the assay was 100%. The combined positive and negative predictive values of the assay were 100% (171/171) and 98.9% (185/187), respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 78, Issue 4, April 2014, Pages 338-342
Journal: Diagnostic Microbiology and Infectious Disease - Volume 78, Issue 4, April 2014, Pages 338-342
نویسندگان
Melissa K. Deck, Erica S. Anderson, Rebecca J. Buckner, Georgia Colasante, Thomas E. Davis, James M. Coull, Benjamin Crystal, Phyllis Della Latta, Martin Fuchs, Deanna Fuller, Will Harris, Kevin Hazen, Lisa L. Klimas, Daniel Lindao, Michelle C. Meltzer,