کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6116490 | 1215962 | 2012 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of the performance of 5 commercialized enzyme immunoassays for the detection of Taenia solium antibodies and for the diagnosis of neurocysticercosis
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Evaluation of the performance of 5 commercialized enzyme immunoassays for the detection of Taenia solium antibodies and for the diagnosis of neurocysticercosis Evaluation of the performance of 5 commercialized enzyme immunoassays for the detection of Taenia solium antibodies and for the diagnosis of neurocysticercosis](/preview/png/6116490.png)
چکیده انگلیسی
This study aimed to evaluate 5 enzyme immunoassays for detecting human antibodies against Taenia solium in human serum and for the diagnosis of neurocysticercosis (NCC): DRGâ¢, RIDASCREENâ¢, NOVATECHâ¢, CYPRESSâ¢, and IVDâ¢. A collection of 114 reference serum samples were used. All sera were tested both by ELISA and by an immunoblot method (enzyme-linked immunoelectrotransfer blot [EITB]). When compared with EITB, the Ridascreen⢠test had the best positive concordance rate (85.1-91.2%) and the NovaLisa test⢠showed the optimal negative concordance rate (93.7-95.6%). All tests had a sensitivity under 72% and a specificity above 60%. The best sensitivity was obtained using Ridascreen⢠test (71.4%). An optimal specificity was achieved by the NovaLisa testâ¢. T. solium-positive sera all cross-reacted with E. granulosus positive samples. In the commercial assays evaluated here, the most appropriate ELISA test for screening may be the Ridascreen⢠assay. Antibody detection seems to be not appropriate for NCC diagnosis because of its overall lack of sensitivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 72, Issue 1, January 2012, Pages 85-89
Journal: Diagnostic Microbiology and Infectious Disease - Volume 72, Issue 1, January 2012, Pages 85-89
نویسندگان
Jean-François Carod, Mickaël Randrianarison, Julien Razafimahefa, Rondro Mamitiana Ramahefarisoa, Mahenintsoa Rakotondrazaka, Monique Debruyne, Mélanie Dautigny, Pierrette Cazal, Marcellin Lalaoarisoa Andriantseheno, Emile Ramarokoto Charles,