کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6118189 | 1591804 | 2012 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Expression of multidrug resistance efflux pump genes in clinical and environmental isolates of Staphylococcus aureus Expression of multidrug resistance efflux pump genes in clinical and environmental isolates of Staphylococcus aureus](/preview/png/6118189.png)
Increased expression of multidrug resistance efflux pump (MDR-EP) genes in clinical isolates of Staphylococcus aureus occurs frequently, but its temporal and geographic variability is unknown. Such strains may contaminate the hospital environment, posing an infection control problem. Nearly 700 clinical isolates from different geographic locales as well as 91 environmental isolates recovered from two Detroit hospitals were studied. Ethidium bromide (EtBr) minimum inhibitory concentration (MIC), quantitative expression of all characterised chromosomal MDR-EP genes, and the presence of qacA/B and smr were determined for all strains. In addition, for norA- and/or mepA-overexpressing strains, the spa type was established. MDR-EP gene overexpression varied temporally and geographically, and overexpressing strains were present in the hospital environment. Increased expression of norA was associated with meticillin resistance and spa type t002, a rare type among control strains, consistent with widespread dissemination of a norA-overexpressing, meticillin-resistant S. aureus (MRSA) clone. Clonal spread also played a role for spa type t008, mepA-overexpressing, meticillin-susceptible strains. An EtBr MIC of â¤12.5 μg/mL was highly specific (>90%) in identifying strains lacking MDR-EP gene overexpression.
Journal: International Journal of Antimicrobial Agents - Volume 40, Issue 3, September 2012, Pages 204-209