کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6124139 1219962 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular typing and in-vitro activity of azoles against clinical isolates of Aspergillus fumigatus and A. niger in Japan
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Molecular typing and in-vitro activity of azoles against clinical isolates of Aspergillus fumigatus and A. niger in Japan
چکیده انگلیسی
Twenty-three isolates of Aspergillus from Japanese transplant recipients were identified by phenotyping and subjected to detailed sequence analyses using the ITS regions and β-tubulin (benA). Gene sequences derived from the ITS regions and benA of all Aspergillus isolates were compared with sequences in the GenBank database for species identification. Nineteen isolates were identified as A. fumigatus and 4 isolates as A. niger. The antifungal susceptibility of the isolates to itraconazole (ITZ), voriconazole (VCZ), and posaconazole (POS) was assessed using the E-test with a 48-h incubation. Almost all the clinical isolates of the Aspergillus species seemed to be more susceptible to VCZ and POS than to ITZ, whereas 1 clinical isolate of A. fumigatus was resistant to ITZ. Sequencing of the 14α-demethylase gene (cyp51A) of this isolate revealed a novel mutation (F332K) in the gene.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Infection and Chemotherapy - Volume 17, Issue 4, 2011, Pages 483-486
نویسندگان
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