کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6170755 1251415 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Engineering and characterization of simplified vaginal and seminal fluid simulants
ترجمه فارسی عنوان
مهندسی و ویژگی ساده سازی واژینال و مایع مخلوط مایع
کلمات کلیدی
مایع سرویکوواژینال انسان، ظرفیت بافری، طراحی شبیه ساز، سمیت مسمومیت،
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
چکیده انگلیسی

BackgroundReported vaginal and seminal fluid simulants have complex compositions with multiple preparatory steps that contribute to physical instability. We report the design and characterization of stable and simplified buffers that mimic the salient physical/chemical properties of the physiological fluids.Study design/methodsHuman cervicovaginal and seminal fluid samples were collected and buffering capacity was determined. The major buffering species were identified from published compositions of reproductive tract fluids. These values were used to compute the composition of vaginal and seminal fluid simulants. Ionic strength, buffering capacities, pH and osmolalities were then calculated or experimentally determined. Finally, cytotoxicity was evaluated in HEC-1-A cells and 3D reconstructed EpiVaginal™ tissue (VEC-100-FT) using naïve cells/tissue and nonoxynol-9 as controls.ResultsThe use of calculated amounts of conjugate acid and base for buffer development resulted in compositions that did not require endpoint pH adjustment and could be formulated as stable 10 × concentrates. Furthermore, due to the absence of complex divalent salts, all our proposed simulants were stable at 4°C for 1 month whereas precipitation and pH and osmolality changes were noted in reported buffers. Experimental determination of buffering capacities yielded similar values for undiluted cervicovaginal fluid (β4.2-5.2= 35.6±12.3 mM, N= 7) and human seminal fluid (β7 -6= 37.5±5 mM, N= 3). All neat simulants showed significant cytotoxicity in HEC-1-A cells but were well tolerated by organotypic vaginal tissue.ConclusionsWe report revised and improved compositions of buffers mimicking salient properties of vaginal and seminal fluid necessary for in vitro product evaluation.ImplicationsTo support research in reproductive health and in particular drug delivery, we have designed and characterized stable new media to mimic these important fluids that can be used in a variety of in vitro studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Contraception - Volume 93, Issue 4, April 2016, Pages 337-346
نویسندگان
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