کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6196672 1602589 2015 19 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
From nano to macro: Studying the hierarchical structure of the corneal extracellular matrix
ترجمه فارسی عنوان
از نانو به ماکرو: مطالعه ساختار سلسله مراتبی ماتریکس خارج سلولی قرنیه
کلمات کلیدی
زیست شناسی ساختاری، پراکندگی اشعه ایکس، میکروسکوپ نوری غیر خطی، نسل دوم هارمونیک، کلاژن، قرنیه ماتریکس خارج سلولی،
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
چکیده انگلیسی


- Methods used to characterize the hierarchical assembly of the ECM are discussed.
- 3-D analysis of ECM structure by TEM/SEM and NLO SHG microscopy is presented.
- New insights into the structural blueprint of the cornea are uncovered.

In this review, we discuss current methods for studying ocular extracellular matrix (ECM) assembly from the 'nano' to the 'macro' levels of hierarchical organization. Since collagen is the major structural protein in the eye, providing mechanical strength and controlling ocular shape, the methods presented focus on understanding the molecular assembly of collagen at the nanometre level using X-ray scattering through to the millimetre to centimetre level using non-linear optical (NLO) imaging of second harmonic generated (SHG) signals. Three-dimensional analysis of ECM structure is also discussed, including electron tomography, serial block face scanning electron microscopy (SBF-SEM) and digital image reconstruction. Techniques to detect non-collagenous structural components of the ECM are also presented, and these include immunoelectron microscopy and staining with cationic dyes. Together, these various approaches are providing new insights into the structural blueprint of the ocular ECM, and in particular that of the cornea, which impacts upon our current understanding of the control of corneal shape, pathogenic mechanisms underlying ectatic disorders of the cornea and the potential for corneal tissue engineering.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 133, April 2015, Pages 81-99
نویسندگان
, , , , , , ,