A detailed three-step protocol for live imaging of intracellular traffic in polarized primary porcine RPE monolayers

- We present a detailed 3-step protocol for live imaging of polarized primary RPE.
- Step 1: a protocol for primary porcine retinal pigment epithelial culture.
- This method results in highly polarized monolayers within 7 days.
- Step 2: two gene delivery protocols that yield efficient transgene expression.
- Step 3: High speed live imaging to visualize intracellular traffic in the RPE.