کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6197502 1261174 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
GABA agonists fail to protect the retina from ischemia-reperfusion injury
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
پیش نمایش صفحه اول مقاله
GABA agonists fail to protect the retina from ischemia-reperfusion injury
چکیده انگلیسی

The purpose of this study was to test the hypothesis that ischemia/reperfusion injury in the rat retina may be ameliorated by reducing retinal metabolism with either hypothermia or inhibitory GABA agonists. The intraocular pressure of each right eye in rats was raised to 130 mmHg for 60 min with the left eye serving as normal control. The rats were divided into four groups in terms of drug and hypothermia treatment: (1) Untreated ischemia, (2) Hypothermia, (3) Baclofen/midazolam and (4) Baclofen/muscimol. Electroretinogram was recorded before ischemia and again after 10-day reperfusion. Histological analysis with H&E staining and cell counts was performed. Untreated ischemia/reperfusion resulted in severely reduced ERG responses. The ERG b-wave was reduced from 423 ± 144 μV to 130 ± 91 μV (mean ± SD, n = 5). With hypothermia the ERG b-wave was reduced from 499 ± 80 μV to 237 ± 111 μV (n = 4). With combinations of baclofen and midazolam the ERG b-wave was reduced from 432 ± 96 μV to 104 ± 67 μV (n = 7). In baclofen/muscimol treated eyes the ERG b-wave went from 426 ± 101 μV to 148 ± 118 μV (n = 6). The histological tissue damage was severe in untreated ischemia and the baclofen/midazolam and baclofen/muscimol groups, but less severe in the hypothermia group. The GABA agonists do not provide any protection in our ischemia/reperfusion model. Our results are consistent with earlier reports that hypothermia may be helpful in ischemic conditions in the retina.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 88, Issue 3, March 2009, Pages 361-366
نویسندگان
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