Population balance modelling of stem cell culture in 3D suspension bioreactors

- Mathematical model developed for ESC 3D suspension culture.
- Modelled effects of varying substrate and gas concentration on total cell number.
- Model behaviour shown to be consistent with literature data.
- High initial substrate, O2, fed-batch system maximise total cell number.
- Proof-of-concept model for optimisation of stem cell culture in 3D.