Effect of different extraction methods on bound EPS from MBR sludges. Part I: Influence of extraction methods over three-dimensional EEM fluorescence spectroscopy fingerprint

With regard to the study using excitation-emission matrix (EEM) fluorescence spectroscopy to characterize the fluorescence signals of EPS, the fluorescence spectra showed the presence of protein-derived compounds in all EPS extracted. Additionally, humic-like substances were found when formaldehyde was used in the EPS extraction. Also, it was found that the peak intensity strongly depended on the EPS concentration.