کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6261594 | 1613232 | 2016 | 10 صفحه PDF | دانلود رایگان |
- i.p. 50Â mg/kg Guo decreased the number of spike-wave discharges (SWDs).
- i.p. 100Â mg/kg Guo enhanced the number of SWDs in WAG/Rij rats.
- Effect of 100Â mg/kg Guo was inhibited by theophylline, SCH 58261 and indomethacin.
- i.p. 100Â mg/kg Guo may increase SWD number by means of adenosinergic system.
The non-adenosine nucleoside guanosine (Guo) was demonstrated to decrease quinolinic acid(QA)-induced seizures, spontaneously emerged absence epileptic seizures and lipopolysaccharide(LPS)-evoked induction of absence epileptic seizures suggesting its antiepileptic potential. It was also described previously that intraperitoneal (i.p.) injection of 20 and 50Â mg/kg Guo decreased the number of spike-wave discharges (SWDs) in a well investigated model of human absence epilepsy, the Wistar Albino Glaxo Rijswijk (WAG/Rij) rats during 4th (20Â mg/kg Guo) and 3rd as well as 4th (50Â mg/kg Guo) measuring hours. Guanosine can potentially decrease SWD number by means of its putative receptors but absence epileptic activity changing effects of Guo by means of increased extracellular adenosine (Ado) cannot be excluded. An increase in the dose of i.p. injected Guo is limited by its low solubility in saline, therefore, we addressed in the present study whether higher doses of Guo, diluted in sodium hydroxide (NaOH) solution, have more potent antiepileptic effect in WAG/Rij rats. We confirmed that i.p. 50Â mg/kg Guo decreased but, surprisingly, i.p. 100Â mg/kg Guo enhanced the number of SWDs in WAG/Rij rats. Combined i.p. injection of a non-selective Ado receptor antagonist theophylline (5Â mg/kg) or a selective Ado A2A receptor (A2AR) antagonist SCH 58261 (7-(2-phenylethyl)-5-amino-2-(2-furyl)-pyrazolo-[4,3-e]-1,2,4-triazolo[1,5-c]pyrimidine) (1Â mg/kg) and a cyclooxygenase 1 and 2/COX-1 and COX-2 inhibitor indomethacin (10Â mg/kg) with 100Â mg/kg Guo decreased the SWD number compared to i.p. 100Â mg/kg Guo alone. The results suggest that i.p. 100Â mg/kg Guo can increase SWD number by means of the adenosinergic system.
Journal: Brain Research Bulletin - Volume 124, June 2016, Pages 172-181