کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6261916 1613266 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research reportMitochondrial dysfunction in human TDP-43 transfected NSC34 cell lines and the protective effect of dimethoxy curcumin
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
Research reportMitochondrial dysfunction in human TDP-43 transfected NSC34 cell lines and the protective effect of dimethoxy curcumin
چکیده انگلیسی

TAR-DNA-binding protein of 43 kDa (TDP-43) was recently found to be one of the major disease proteins in the pathological inclusions of amyotrophic lateral sclerosis (ALS). The effect of TDP-43 on mitochondrial function remains poorly understood. Here, we show that human TDP-43 caused mitochondrial morphologic abnormality, decrease of mitochondrial complex I activity and mitochondrial transmembrane potential, and increased expression of mitochondrial uncoupling protein 2 (UCP2) in human TDP-43 stably transfected NSC-34 cells by using flow cytometric analysis, spectrophotometric assays, electron microscopy and Western blotting. We also show that dimethoxy curcumin (DMC) could ameliorate mitochondrial dysfunction in mutated TDP-43 stably transfected cell lines. DMC could be potentially useful for neurodegenerative diseases linked with mutated TDP-43.

► Explored mitochondrial dysfunction caused by TDP-43 in pathogenesis of ALS. ► Found mitochondrial complex I could be one of pathogenic targets of TDP-43. ► Observed protective effect of dimethoxy curcumin against mitochondrial dysfunction in TDP-43 transfected cells. ► Proposed that dimethoxy curcumin could be a potential therapy approach in neurodegenerative diseases linked with mutated TDP-43.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research Bulletin - Volume 89, Issues 5–6, 1 December 2012, Pages 185-190
نویسندگان
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