کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6262006 | 1290787 | 2012 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Research reportNeuroprotection of co-activation of GABA receptors by preventing caspase-3 denitrosylation in KA-induced seizures Research reportNeuroprotection of co-activation of GABA receptors by preventing caspase-3 denitrosylation in KA-induced seizures](/preview/png/6262006.png)
Previous studies have demonstrated that kainic acid (KA)-induced seizures can cause the enhancement of excitation and lead to neuronal death in rat hippocampus. Co-activation of the inhibitory GABA receptors can attenuate the excitatory JNK3 apoptotic signaling pathway via inhibiting the increased assembly of the GluR6-PSD-95-MLK3 signaling module induced by KA in epileptic rat hippocampal CA1 and CA3 regions. Caspase-3 is a cysteine protease located in both the cytoplasm and mitochondrial intermembrane space that is a central effector of many apoptotic pathways. We designed experiments to elucidate the underlying molecular mechanisms of procaspase-3 activation and neuroprotection of co-activation of GABA receptors against neuronal death induced by KA. In this study, we show that co-activation of GABA receptors can attenuate the Fas/FasL apoptotic signaling pathway and inhibit the increased of thioredoxin reductase activity induced by KA, subsequently inhibit the activation of procaspase-3 by diminishing the denitrosylation of its active-site thiol and decreasing the cleavage of the caspase-3 zymogen to its active subunits. These results indicate that co-activation of GABA receptors results in neuroprotection by preventing caspase-3 denitrosylation in KA-induced seizure of rats.
⺠Co-activation of GABA receptors attenuates the Fas/FasL apoptotic signaling pathway. ⺠Co-activation of GABA receptors inhibits the increasing of thioredoxin reductase activity. ⺠Co-activation of GABA receptors inhibits the activation of procaspase-3.
Journal: Brain Research Bulletin - Volume 88, Issue 6, 1 September 2012, Pages 617-623