کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6263505 1613907 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research reportEarly postnatal GFAP-expressing cells produce multilineage progeny in cerebrum and astrocytes in cerebellum of adult mice
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Research reportEarly postnatal GFAP-expressing cells produce multilineage progeny in cerebrum and astrocytes in cerebellum of adult mice
چکیده انگلیسی


- Exogenous tamoxifen induces Cre recombinase expression in hGfapCreERT2 mice.
- Temporal specificity of Cre is achieved by controlling the timing of tamoxifen use.
- The early postnatal GFAP-positive cells possess the NSC attributes in vivo.
- In cerebrum, these cells generate astrocytes, neurons and oligodendrocytes.
- In adult mouse cerebellum, these cells only differentiate into astrocytes.

Early postnatal GFAP-expressing cells are thought to be immature astrocytes. However, it is not clear if they possess multilineage capacity and if they can generate different lineages (astrocytes, neurons and oligodendrocytes) in the brain of adult mice.In order to identify the fate of astroglial cells in the postnatal brain, hGFAP-Cre-ERT2 transgenic mice were crossed with the R26R Cre reporter mouse strains which exhibit constitutive expression of β-galactosidase (β-gal). Mice carrying the hGFAP-Cre-ERT2/R26R transgene were treated with Tamoxifen to induce Cre recombination in astroglial cells at postnatal (P) day 6 and Cre recombinase-expressing cells were identified by X-gal staining. Immunohistochemical staining was used to identify the type(s) of these reporter-tagged cells. Sixty days after recombination, X-gal-positive cells in different cerebral regions of the adult mice expressed the astroglial markers Blbp and GFAP, the neuronal marker NeuN, the oligodendrocyte precursor cell marker NG2 and the mature oligodendrocyte marker CC1. X-gal-positive cells in the cerebellum coexpressed the astroglial marker Blbp, but not the granule cell marker NeuN, Purkinje cell marker Calbindin or oligodendrocyte precursor cell marker NG2.Our genetic fate mapping data demonstrated that early postnatal GFAP-positive cells possessed multilineage potential and eventually differentiated into neurons, astrocytes, and oligodendrocyte precursor cells in the cerebrum and into astrocytes (including Bergmann glia) in the cerebellum of adult mice.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1532, 26 September 2013, Pages 14-20
نویسندگان
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