کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6265363 1614070 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research ReportSubstance P induces the reversible formation of varicosities in the dendrites of rat brainstem neurons
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Research ReportSubstance P induces the reversible formation of varicosities in the dendrites of rat brainstem neurons
چکیده انگلیسی

This study investigated the ability of substance P (Sub P) to induce dendritic varicosities (DVs) or beads in neurons of the rostral ventromedial medulla (RVM) of the rat. Microinjection of 5-200 pmol Sub P in the RVM produced a concentration-dependent increase in the number of DVs in distal dendrites of RVM neurons that were immunoreactive for the neurokinin-1 receptor, but not serotonin. The effect was reversible, as DVs were essentially absent 2 and 4 h after microinjection. Fluoro-Jade B labeled neurons were not evident in the RVM 4 days after microinjection of Sub P, although such neurons were present 4 days after microinjection of a neurotoxic dose of kainate. Bath application of Sub P to brainstem slices for a period as brief as 30 s also produced DVs in neurokinin-1 immunoreactive RVM neurons. Prior exposure to L-703606 prevented the formation of DVs by Sub P, implicating the neurokinin-1 receptor, a Gq type of G protein coupled receptor, in the formation of DVs by Sub P. Finally, stabilization of microtubules by prior exposure to taxol also prevented the formation of DVs, consistent with the idea that increases in intracellular Ca2+ lead to the formation of DVs secondary to a disruption of the linear arrays of microtubules in dendrites. These data establish a mechanistic basis for the formation of DVs by Sub P and support further studies to test the hypothesis that the formation of DVs is a morphological mechanism by which neurons can regulate their responses to inhibitory or excitatory inputs.

Research Highlights►Substance P produces varicosities and constrictions in dendrites of medullary neurons. ►Substance P evoked formation of varicosities is mediated by neurokinin-1 receptors. ►Dendritic varicosities result from Ca++ influx and disruption of microtubules. ►Dendritic varicosities may be a morphological means to regulate neuron excitability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1369, 19 January 2011, Pages 36-45
نویسندگان
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