Production of a monoclonal antibody, against human α-synuclein, in a subpopulation of C57BL/6J mice, presenting a deletion of the α-synuclein locus

▶ Mice deleted for snca are efficient hosts for producing anti-α-synuclein antibodies. ▶ IgG AS11 recognizes the N-terminus of human α-synuclein. ▶ AS11 detects intraneuronal inclusions containing aggregated α-synuclein. ▶ AS11 stains pathological glial cytoplasmic inclusions in brains of patients with MSA. ▶ AS11 detects pathological forms of α-synuclein in Lewy bodies in patients with PD.