کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6270163 | 1295184 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Combination of diOlistic labeling with retrograde tract tracing and immunohistochemistry
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کلمات کلیدی
TBSMSNFluorogoldVGLUT1Staining techniqueDiI - DIIimmunoreactive - ایمنی فعالBallistic - بالستیکDual labeling - برچسب زدن دوگانهTris-buffered saline - تریس بافر شورtyrosine hydroxylase - تیروزین هیدروکسیلازvesicular glutamate transporter 1 - حمل کننده گلوتامات وزیکولار 1tract tracing - ردیابی تراکتDendritic spine - ستون دندریتیکNeuronal morphology - مورفولوژی نورونmedium spiny neuron - نورون ستون فقرات متوسط
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
Neuronal staining techniques have played a crucial role in the analysis of neuronal function. Several different staining techniques have been developed to allow morphological analyses of neurons. DiOlistic labeling, in which beads are coated with a lipophilic dye and then ballistically ejected onto brain tissue, has recently been introduced as a useful and simple means to label neurons and glia in their entirety. Although diOlistic labeling provides detailed information on the morphology of neurons, combining this approach with other staining methods is a significant advance. We have developed protocols that result in high quality diOlistically- and retrogradely-labeled or diOlistically-immunohistochemically labeled neurons. These dual-label methods require modification of fixation parameters and the restricted use of detergents for tissue permeabilization, and are readily applicable to a wide range of tracers and antibodies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 184, Issue 2, 15 November 2009, Pages 332-336
Journal: Journal of Neuroscience Methods - Volume 184, Issue 2, 15 November 2009, Pages 332-336
نویسندگان
M. Diana Neely, Gregg D. Stanwood, Ariel Y. Deutch,