کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6271463 | 1614760 | 2016 | 58 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Metabotropic glutamate receptors are involved in the detection of IMP and l-amino acids by mouse taste sensory cells
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کلمات کلیدی
AIDA(RS)-1-aminoindan-1,5-dicarboxylic acid(RS)-α-Methylserine-O-phosphateTSCs(RS)-α-cyclopropyl-4-phosphonophenylglycineCPPGMSOPl-AP4mGluR4T1R3T1R1MPGN-methyl-d-aspartic acidmGluRIMPmGluR1HEPESGPCRCTAGFPNMDAMSGGMPDHPG(S)-3,5-dihydroxyphenylglycine - (S) -3،5-دی هیدروکسی فنیل گلیسین4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid - 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acidD-(−)-2-amino-5-phosphonopentanoic acid - D - (-) - 2-amino-5-phosphonopentanoic acidd-AP5 - D-AP5G-protein-coupled receptor - G-پروتئین گیرندهiGluR - Iglul-Serine - L-Serinel-arginine - آرژینین الArg - ارگl-Amino acids - ال-آمینو اسیدهاSER - برای بودنConditioned taste aversion - بی نظمی طعم و مزهhuman embryonic kidney cells - سلول های کلیوی جنینی انسانHEK cells - سلولهای HEKCNQX - سیانکیوایکسTaste - مزهgreen fluorescent protein - پروتئین فلورسنت سبزmonosodium glutamate - گلوتامات monosodiumMetabotropic glutamate receptor - گیرنده گلوتامات متابوتروپیکionotropic glutamate receptor - گیرنده ی گلوتامات ionotropic
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
G-protein-coupled receptors are thought to be involved in the detection of umami and l-amino acid taste. These include the heterodimer taste receptor type 1 member 1 (T1r1) + taste receptor type 1 member 3 (T1r3), taste and brain variants of mGluR4 and mGluR1, and calcium sensors. While several studies suggest T1r1 + T1r3 is a broadly tuned l-amino acid receptor, little is known about the function of metabotropic glutamate receptors (mGluRs) in l-amino acid taste transduction. Calcium imaging of isolated taste sensory cells (TSCs) of T1r3-GFP and T1r3 knock-out (T1r3 KO) mice was performed using the ratiometric dye Fura 2 AM to investigate the role of different mGluRs in detecting various l-amino acids and inosine 5Ⲡmonophosphate (IMP). Using agonists selective for various mGluRs such as (RS)-3,5-dihydroxyphenylglycine (DHPG) (an mGluR1 agonist) and l-(+)-2-amino-4-phosphonobutyric acid (l-AP4) (an mGluR4 agonist), we evaluated TSCs to determine if they might respond to these agonists, IMP, and three l-amino acids (monopotassium l-glutamate, l-serine and l-arginine). Additionally, we used selective antagonists against different mGluRs such as (RS)-1-aminoindan-1,5-dicarboxylic acid (AIDA) (an mGluR1 antagonist), and (RS)-α-methylserine-O-phosphate (MSOP) (an mGluR4 antagonist) to determine if they can block responses elicited by these l-amino acids and IMP. We found that l-amino acid- and IMP-responsive cells also responded to each agonist. Antagonists for mGluR4 and mGluR1 significantly blocked the responses elicited by IMP and each of the l-amino acids. Collectively, these data provide evidence for the involvement of taste and brain variants of mGluR1 and mGluR4 in l-amino acid and IMP taste responses in mice, and support the concept that multiple receptors contribute to IMP and l-amino acid taste.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 316, 1 March 2016, Pages 94-108
Journal: Neuroscience - Volume 316, 1 March 2016, Pages 94-108
نویسندگان
S. Pal Choudhuri, R.J. Delay, E.R. Delay,